Novel flavanones with anti-trypanosomal activity isolated from Zambian and Tanzanian propolis samples

Alenezi, S. S., Natto, M. J., Igoli, J. O., Gray, A. I., Fearnley, J., Fearnley, H., De Koning, H. P. and Watson, D. G. (2020) Novel flavanones with anti-trypanosomal activity isolated from Zambian and Tanzanian propolis samples. International Journal for Parasitology: Drugs and Drug Resistance, 14, pp. 201-207. (doi: 10.1016/j.ijpddr.2020.10.011) (PMID:33160277) (PMCID:PMC7649109)

[img] Text
229818.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

1MB

Abstract

A bioassay-guided phytochemical investigation of propolis samples from Tanzania and Zambia that screened for activity against Trypanosoma brucei has led to the isolation of two novel flavanones with promising antitrypanosomal activity. The compounds were characterized based on their spectral and physical data and identified as 6-(1,1-dimethylallyl) pinocembrin and 5-hydroxy-4″,4″-dimethyl-5″-methyl-5″-H-dihydrofuranol [2″,3″,6,7] flavanone. The two compounds, together with the propolis extracts and fractions, were assayed against a standard drug-sensitive strain of T. b. brucei (s427 wild-type), multi-drug resistant-resistant T. b. brucei (B48), drug-sensitive T. congolense (1L300) and a derived diminazene-resistant T. congolense strain (6C3), and for toxicity against U947 human cells and RAW 246.7 murine cells. Activity against T. b. brucei was higher than against T. congolense. Interestingly, the Tanzanian propolis extract was found to be more active than its fractions and purified compounds in these assays, with an IC of 1.20 μg/mL against T. b. brucei. The results of a cytotoxicity assay showed that the propolis extracts were less toxic than the purified compounds with mean IC values > 165.0 μg/mL.

Item Type:Articles
Additional Information:The work was funded through a PhD studentship from the Ministry of Health State of Kuwait. The support of the Saudi Arabian Ministry of Health for support of MJN is gratefully acknowledged.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:De Koning, Professor Harry and Natto, Dr Manal
Authors: Alenezi, S. S., Natto, M. J., Igoli, J. O., Gray, A. I., Fearnley, J., Fearnley, H., De Koning, H. P., and Watson, D. G.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:International Journal for Parasitology: Drugs and Drug Resistance
Publisher:Elsevier
ISSN:2211-3207
ISSN (Online):2211-3207
Published Online:31 October 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in International Journal for Parasitology: Drugs and Drug Resistance 14:201-207
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record