Domestication of Campylobacter jejuni NCTC 11168

Pascoe, B. et al. (2019) Domestication of Campylobacter jejuni NCTC 11168. Microbial Genomics, 5(7), 000279. (doi: 10.1099/mgen.0.000279) (PMID:31310201) (PMCID:PMC6700657)

Abstract

Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C . jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

• Item Type: Articles
• Additional Information: B. P. and S. K. S. are supported by a Medical Research Council grant (MR/L015080/1). L. K. W. is funded by BBSRC (BB/M009610/1).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Everest, Dr Paul

Creator Roles

Everest, P.: Resources

• Authors: Pascoe, B., Williams, L. K., Calland, J. K., Meric, G., Hitchings, M. D., Dyer, M., Ryder, J., Shaw, S., Lopes, B. S., Chintoan-Uta, C., Allan, E., Vidal, A., Fearnley, C., Everest, P., Pachebat, J. A., Cogan, T. A., Stevens, M. P., Humphrey, T. J., Wilkinson, T. S., Cody, A. J., Colles, F. M., Jolley, K. A., Maiden, M. C. J., Strachan, N., Pearson, B. M., Linton, D., Wren, B. W., Parkhill, J., Kelly, D. J., van Vliet, A. H. M., Forbes, K. J., and Sheppard, S. K.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Microbial Genomics
• Publisher: Microbiology Society
• ISSN: 2057-5858
• ISSN (Online): 2057-5858
• Published Online: 01 July 2019
• Copyright Holders: Copyright © 2019 The Authors
• First Published: First published in Microbial Genomics 5(7): 000279
• Publisher Policy: Reproduced under a Creative Commons License