Validation of a method for collection and assay of pentane in the exhaled breath of the horse

Wyse, C.A., Love, S., Christley, R.M., Yam, P.S., Cooper, J.M., Cumming, D.R.S. and Preston, T. (2004) Validation of a method for collection and assay of pentane in the exhaled breath of the horse. Research in Veterinary Science, 76(2), 109 -112. (doi: 10.1016/j.rvsc.2003.08.007)

Abstract

Oxidative stress refers to an imbalance between the production of oxidising free radicals and the antioxidant defenses of the cell, and is associated with many pathogenic processes. Oxidative damage to cellular lipids results in the evolution of pentane and ethane gas, and detection of these hydrocarbons in the exhaled breath can be used to monitor in vivo oxidative stress. The aim of this study was to validate a gas chromatography (GC) method for measurement of breath pentane in the horse. The GC-system developed showed good specificity for discrimination of pentane from other breath hydrocarbons, and was sensitive to 0.5 ppb pentane. Pentane was detected in the exhaled breath of five horses investigated on two occasions. The results of this preliminary study demonstrate that breath pentane can be measured in the horse, and further work is now justified to investigate the feasibility of applying this method for monitoring in vivo oxidative stress in the horse.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Wyse, Dr Cathy and Preston, Professor Tom and Yam, Dr Philippa and Love, Professor Sandy
• Authors: Wyse, C.A., Love, S., Christley, R.M., Yam, P.S., Cooper, J.M., Cumming, D.R.S., and Preston, T.
• Subjects: S Agriculture > SF Animal culture > SF600 Veterinary Medicine; Q Science > QL Zoology
• College/School: College of Science and Engineering > Scottish Universities Environmental Research Centre; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Research in Veterinary Science
• ISSN: 0034-5288