Confocal and multiphoton imaging of intracellular Ca2+.

Smith, G. , Reynolds, M., Burton, F. and Kemi, O. J. (2010) Confocal and multiphoton imaging of intracellular Ca2+. In: Whitaker, M. (ed.) Calcium in Living Cells. Series: Methods in cell biology (99). Elsevier, pp. 225-261. ISBN 9780123748416 (doi: 10.1016/B978-0-12-374841-6.00009-8)

Abstract

This chapter compares the imaging capabilities of a range of systems including multiphoton microscopy in regard to measurements of intracellular Ca2+ within living cells. In particular, the excitation spectra of popular fluorescent Ca2+ indicators are shown during 1P and 2P excitation. The strengths and limitations of the current indicators are discussed along with error analysis which highlights the value of matching the Ca2+ affinity of the dye to a particular aspect of Ca2+ signaling. Finally, the combined emission spectra of Ca2+ and voltage sensitive dyes are compared to allow the choice of the optimum combination to allow simultaneous intracellular Ca2+ and membrane voltage measurements.

• Item Type: Book Sections
• Status: Published
• Glasgow Author(s) Enlighten ID: Smith, Professor Godfrey and Kemi, Dr Ole and Burton, Dr Francis
• Authors: Smith, G., Reynolds, M., Burton, F., and Kemi, O. J.
• Subjects: Q Science > QP Physiology
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health; College of Medical Veterinary and Life Sciences > School of Life Sciences
• Publisher: Elsevier
• ISBN: 9780123748416