Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry

Doberstein, S.K., Wiegand, G., Machesky, L.M. and Pollard, T.D. (1995) Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry. Cytometry, 20(1), pp. 14-18. (doi: 10.1002/cyto.990200104) (PMID:7600895)

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Abstract

We report here a quick and inexpensive method for preparing standards of known fluorochrome content for calibration and quantitation of flow cytometry fluorescence signals. Erythrocyte ghosts prepared by hypotonic lysis are filled with solutions containing fluorescently labeled dextran. Standards prepared by this technique have a narrow range of fluorescence and a linear response of fluorescence to fluorochrome content up to 2 × 106 fluorochrome molecules/cell. The volume of ghost standard particles is roughly 70 femtoliters (fl)/cell. The fluorescence of ghost standards is nearly identical to that of commercially available microbead standards of similar fluorochrome content. Ghost standards have stable fluorescence for at least 3 weeks at 4°C. These standards can be made with any fluorochrome or combination of fluorochromes over a wide concentration range.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Machesky, Professor Laura
Authors: Doberstein, S.K., Wiegand, G., Machesky, L.M., and Pollard, T.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Cytometry
Publisher:Wiley
ISSN:0196-4763
ISSN (Online):1097-0320

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