Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcγR, phagocytosis

Olazabal, I. M., Caron, E., May, R. C., Schilling, K., Knecht, D. A. and Machesky, L. M. (2002) Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcγR, phagocytosis. Current Biology, 12(16), pp. 1413-1418. (doi: 10.1016/S0960-9822(02)01069-2) (PMID:12194823)

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Abstract

Phagocytosis through Fcγ receptor (FcγR) or complement receptor 3 (CR) requires Arp2/3 complex-mediated actin polymerization, although each receptor uses a distinct signaling pathway [1]. Rac and Cdc42 are required for actin and Arp2/3 complex recruitment during FcγR phagocytosis, while Rho controls actin assembly at CR phagosomes 2, 3. To better understand the role of Rho in CR phagocytosis, we tested the idea that a known target of Rho, Rho-kinase (ROK), might control phagocytic cup formation and/or engulfment of particles. Inhibitors of ROK (dominant-negative ROK and Y-27632) and of the downstream target of ROK, myosin-II (ML7, BDM, and dominant-negative myosin-II), were used to test this idea. We found that inhibition of the Rho → ROK → myosin-II pathway caused a decreased accumulation of Arp2/3 complex and F-actin around bound particles, which led to a reduction in CR-mediated phagocytic engulfment. FcγR-mediated phagocytosis, in contrast, was independent of Rho or ROK activity and was only dependent on myosin-II for particle internalization, not for actin cup formation. While myosins have been previously implicated in FcγR phagocytosis 4, 5, 6, to our knowledge, this is the first demonstration of a role for myosin-II in CR phagocytosis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Machesky, Professor Laura and Knecht, Prof David
Authors: Olazabal, I. M., Caron, E., May, R. C., Schilling, K., Knecht, D. A., and Machesky, L. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Current Biology
Publisher:Elsevier (Cell Press)
ISSN:0960-9822
ISSN (Online):1879-0445
Published Online:25 August 2002

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