Kästele, V. et al. (2021) Intestinal-derived ILCs migrating in lymph increase IFNγ production in response to Salmonella Typhimurium infection. Mucosal Immunology, 14(3), pp. 717-727. (doi: 10.1038/s41385-020-00366-3) (PMID:33414524) (PMCID:PMC8075955)
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Abstract
Innate lymphoid cells (ILCs) are enriched in mucosae and have been described as tissue-resident. Interestingly, ILCs are also present within lymph nodes (LNs), in the interfollicular regions, the destination for lymph-migratory cells. We have previously shown that LN ILCs are supplemented by peripheral tissue-derived ILCs. Using thoracic duct cannulations, we here enumerate the intestinal lymph ILCs that traffic from the intestine to the mesenteric LNs (MLNs). We provide, for the first time, a detailed characterisation of these lymph-migratory ILCs. We show that all ILC subsets migrate in lymph, and while global transcriptional analysis reveals a shared signature with tissue-resident ILCs, lymph ILCs express migration-associated genes including S1PRs, SELL (CD62L) and CCR7. Interestingly, we discovered that while Salmonella Typhimurium infections do not increase the numbers of migrating ILCs, infection changes their composition and cytokine profile. Infection increases the proportions of RORyt+ T-bet+ ILCs, levels of IFNγ, and IFNγ/GM-CSF co-expression. Infection-induced changes in migratory ILCs are reflected in colon-draining MLN ILCs, where RORyt+ T-bet+ ILCs accumulate and display corresponding increased cytokine expression. Thus, we reveal that ILCs respond rapidly to intestinal infection and can migrate to the MLN where they produce cytokines.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Mayer, Mr Johannes and Cole, Mr John and Kaestele, Ms Verena and Maciewicz, Dr Rose and Milling, Professor Simon and Wall, Dr Daniel and Goodyear, Professor Carl |
Authors: | Kästele, V., Mayer, J., Lee, E. S., Papazian, N., Cole, J. J., Cerovik, V., Belz, G., Tomura, M., Eberl, G., Goodyear, C., Maciewicz, R. A., Wall, D., Cupedo, T., Withers, D. R., and Milling, S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
Journal Name: | Mucosal Immunology |
Publisher: | Nature Research |
ISSN: | 1933-0219 |
ISSN (Online): | 1935-3456 |
Published Online: | 07 January 2021 |
Copyright Holders: | Copyright © 2021 The Authors |
First Published: | First published in Mucosal Immunology 14(3): 717-727 |
Publisher Policy: | Reproduced under a Creative Commons Licence |
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