Singh, S. P. and Insall, R. H. (2021) Adhesion stimulates Scar/WAVE phosphorylation in mammalian cells. Communicative and Integrative Biology, 14(1), pp. 1-4. (doi: 10.1080/19420889.2020.1855854) (PMID:33447346) (PMCID:PMC7781634)
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Abstract
The Scar/WAVE complex catalyzes the protrusion of pseudopods and lamellipods, and is therefore a principal regulator of cell migration. However, it is unclear how its activity is regulated, beyond a dependence on Rac. Phosphorylation of the proline-rich region, by kinases such as Erk2, has been suggested as an upstream activator. We have recently reported that phosphorylation is not required for complex activation. Rather, it occurs after Scar/WAVE has been activated, and acts as a modulator. Neither chemoattractant signaling nor Erk2 affects the amount of phosphorylation, though in Dictyostelium it is promoted by cell-substrate adhesion. We now report that cell-substrate adhesion also promotes Scar/WAVE2 phosphorylation in mammalian cells, suggesting that the process is evolutionarily conserved.
Item Type: | Articles |
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Additional Information: | This work was supported by Cancer Research UK core grant number [A17196] and Multidisciplinary Award [A20017] to RHI. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Insall, Professor Robert and Singh, Dr Shashi |
Authors: | Singh, S. P., and Insall, R. H. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Communicative and Integrative Biology |
Publisher: | Taylor & Francis |
ISSN: | 1942-0889 |
ISSN (Online): | 1942-0889 |
Published Online: | 28 December 2020 |
Copyright Holders: | Copyright © 2020 The Authors |
First Published: | First published in Communicative and Integrative Biology 14(1): 1-4 |
Publisher Policy: | Reproduced under a Creative Commons License |
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