Adhesion stimulates Scar/WAVE phosphorylation in mammalian cells

Singh, S. P. and Insall, R. H. (2021) Adhesion stimulates Scar/WAVE phosphorylation in mammalian cells. Communicative and Integrative Biology, 14(1), pp. 1-4. (doi: 10.1080/19420889.2020.1855854) (PMID:33447346) (PMCID:PMC7781634)

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The Scar/WAVE complex catalyzes the protrusion of pseudopods and lamellipods, and is therefore a principal regulator of cell migration. However, it is unclear how its activity is regulated, beyond a dependence on Rac. Phosphorylation of the proline-rich region, by kinases such as Erk2, has been suggested as an upstream activator. We have recently reported that phosphorylation is not required for complex activation. Rather, it occurs after Scar/WAVE has been activated, and acts as a modulator. Neither chemoattractant signaling nor Erk2 affects the amount of phosphorylation, though in Dictyostelium it is promoted by cell-substrate adhesion. We now report that cell-substrate adhesion also promotes Scar/WAVE2 phosphorylation in mammalian cells, suggesting that the process is evolutionarily conserved.

Item Type:Articles
Additional Information:This work was supported by Cancer Research UK core grant number [A17196] and Multidisciplinary Award [A20017] to RHI.
Glasgow Author(s) Enlighten ID:Insall, Professor Robert
Authors: Singh, S. P., and Insall, R. H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Communicative and Integrative Biology
Publisher:Taylor & Francis
ISSN (Online):1942-0889
Published Online:28 December 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Communicative and Integrative Biology 14(1): 1-4
Publisher Policy:Reproduced under a Creative Commons License

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