Haston, J.L., Fitzgerald, O., Kane, D. and Smith, K.D. (2003) The influence of alpha(1)-acid glycoprotein on collagenase-3 activity in early rheumatoid arthritis. Biomedical Chromatography, 17, pp. 361-364. (doi: 10.1002/bmc.251)
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Abstract
The concentration and glycosylation of α1-acid glycoprotein (AGP) alter significantly during inflammation. A definitive physiological role for AGP remains elusive and is the subject of extensive investigation. This study investigated the influence of AGP on the activity of collagenase-3, an important mediator of cartilage destruction in rheumatoid arthritis. AGP was isolated from normal and rheumatoid plasma. Fucosylation was determined by high pH anion-exchange chromatography; sialylation was assessed following enzymatic digest. Rheumatoid AGP displayed elevated fucosylation and sialylation compared with normal. The influence of each sample on collagenase-3 activity was measured fluorometrically. AGP influenced collagenase-3 catalysis and collagen binding, with catalytic activity correlating with fucosylation. Rheumatoid AGP exhibited less efficient inhibition than normal plasma AGP. It is hypothesized that AGP within rheumatoid synovial fluid may be inadequate to prevent excessive cartilage destruction and hence may exacerbate the disease process.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Kane, Prof David |
| Authors: | Haston, J.L., Fitzgerald, O., Kane, D., and Smith, K.D. |
| College/School: | College of Medical Veterinary and Life Sciences |
| Journal Name: | Biomedical Chromatography |
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