Tubulointerstitial nephritis antigen-like 1 protein is downregulated in the placenta of pre-eclamptic women

Mary, S. , Kulkarni, M. J., Mehendale, S. S., Joshi, S. R. and Giri, A. P. (2017) Tubulointerstitial nephritis antigen-like 1 protein is downregulated in the placenta of pre-eclamptic women. Clinical Proteomics, 14, 8. (doi: 10.1186/s12014-017-9144-2) (PMID:28344540) (PMCID:PMC5361709)

Abstract

Background: Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction. Research related to TINAGL1 is limited to cell culture and animal models. Demonstration of TINAGL1 as a positive regulator of angiogenesis and its expression in the decidua of postimplantation mouse uterus, prompted us to validate its expression in human placenta during impaired angiogenesis in pre-eclamptic condition. Methods: Placental tissue from normotensive (n = 25) and pre-eclamptic (n = 25) pregnancies were used to study the differentially expressed proteins by two-dimensional gel electrophoresis and TINAGL1 protein was validated with Western blotting. Results: A total of 55 protein spots were differentially expressed (fold change >1.5, p < 0.05), of which 27 were upregulated and 28 were downregulated in the pre-eclamptic placenta. TINAGL1 was found to be downregulated in pre-eclamptic compared to normotensive pregnant women. Conclusion: This is the first study reporting TINAGL1 to be present in human placenta and differentially expressed in pre-eclamptic condition. The functional role of TINAGL1 in association to human pregnancy needs to be explored further.

• Item Type: Articles
• Additional Information: This work was supported by the Department of Biotechnology, India (Grant: GAP290526).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Samji, Dr Sheon
• Authors: Mary, S., Kulkarni, M. J., Mehendale, S. S., Joshi, S. R., and Giri, A. P.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Clinical Proteomics
• Publisher: BioMed Central
• ISSN: 1542-6416
• ISSN (Online): 1559-0275
• Copyright Holders: Copyright © 2017 The Authors
• First Published: First published in Clinical Proteomics 14: 8
• Publisher Policy: Reproduced under a Creative Commons License