Confocal myography for the study of hypertensive vascular remodelling

Arribas, S. M., González, J. M., Briones, A. M., Somoza, B., Daly, C. J. , Vila, E., González, M. C. and McGrath, J. C. (2007) Confocal myography for the study of hypertensive vascular remodelling. Clinical Hemorheology and Microcirculation, 37(1-2), pp. 205-210.

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Publisher's URL: https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch1016

Abstract

Hypertension is associated with vascular structural alterations known as “vascular remodelling”, which initially are adaptive but in the long run, lead to vascular damage and loss of function. Despite decades of study, there is still modest information on the 3-dimensional (3D) arrangement of vascular cells and extracellular matrix (ECM) and how they change under pathological situations. To address this problem we developed a technique which combines fluorescence confocal microscopy, pressure myography and image analysis, “confocal myography”, which permits the study of intact resistance-sized vessels at cellular level and at physiological pressure. With the aid of this method, we have identified, in arteries from hypertensive rats, abnormal orientation of endothelial, smooth muscle cells (SMC) and elastic fibres; elongation and denudation of endothelial cells, and adventitial hypercellularity. Confocal myography offers a new approach to the study of vascular remodelling in intact small arteries from a 3D point of view.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Daly, Dr Craig
Authors: Arribas, S. M., González, J. M., Briones, A. M., Somoza, B., Daly, C. J., Vila, E., González, M. C., and McGrath, J. C.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Clinical Hemorheology and Microcirculation
Publisher:IOS Press
ISSN:1386-0291

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