Povelones, M. L., Tiengwe, C., Gluenz, E. , Gull, K., Englund, P. T. and Jensen, R. E. (2013) Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1. Molecular Microbiology, 87(4), pp. 713-729. (doi: 10.1111/mmi.12089) (PMID:23336702) (PMCID:PMC4715881)
Full text not currently available from Enlighten.
Abstract
In an RNAi library screen for loss of kinetoplast DNA (kDNA), we identified an uncharacterized Trypanosoma brucei protein, named TbLOK1, required for maintenance of mitochondrial shape and function. We found the TbLOK1 protein located in discrete patches in the mitochondrial outer membrane. Knock‐down of TbLOK1 in procyclic trypanosomes caused the highly interconnected mitochondrial structure to collapse, forming an unbranched tubule remarkably similar to the streamlined organelle seen in the bloodstream form. Following RNAi, defects in mitochondrial respiration, inner membrane potential and mitochondrial transcription were observed. At later times following TbLOK1 depletion, kDNA was lost and a more drastic alteration in mitochondrial structure was found. Our results demonstrate the close relationship between organelle structure and function in trypanosomes.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Gluenz, Dr Eva |
Authors: | Povelones, M. L., Tiengwe, C., Gluenz, E., Gull, K., Englund, P. T., and Jensen, R. E. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Molecular Microbiology |
Publisher: | Wiley |
ISSN: | 0950-382X |
ISSN (Online): | 1365-2958 |
Published Online: | 21 January 2013 |
University Staff: Request a correction | Enlighten Editors: Update this record