Chondrobags: a high throughput alginate-fibronectin micromass platform for in vitro human cartilage formation

Witte, K. , de Andrés, M. C., Wells, J., Dalby, M. J. , Salmeron-Sanchez, M. and Oreffo, R. O.C. (2020) Chondrobags: a high throughput alginate-fibronectin micromass platform for in vitro human cartilage formation. Biofabrication, 12(4), 045034. (doi: 10.1088/1758-5090/abb653)

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Abstract

The maintenance and expansion of the cells required for formation of tissue-engineered cartilage has, to date, proven difficult. This is, in part, due to the initial solid phase extracellular matrix demanded by the cells inhabiting this avascular tissue. Herein, we engineer an innovative alginate-fibronectin microfluidic-based carrier construct (termed a chondrobag) equipped with solid phase presentation of growth factors that support skeletal stem cell chondrogenic differentiation while preserving human articular chondrocyte phenotype. Results demonstrate biocompatibility, cell viability, proliferation and tissue-specific differentiation for chondrogenic markers SOX9, COL2A1 and ACAN. Modulation of chondrogenic cell hypertrophy, following culture within chondrobags loaded with TGF-β1, was confirmed by down-regulation of hypertrophic genes COL10A1 and MMP13. MicroRNAs involved in the chondrogenesis process, including miR-140, miR-146b and miR-138 were observed. Results demonstrate the generation of a novel high-throughput, microfluidic-based, scalable carrier that supports human chondrogenesis with significant implications therein for cartilage repair-based therapies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Seyed Mohammadi, Dr Kimia and Dalby, Professor Matthew
Authors: Witte, K., de Andrés, M. C., Wells, J., Dalby, M. J., Salmeron-Sanchez, M., and Oreffo, R. O.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biofabrication
Publisher:Institute of Physics Publishing Ltd.
ISSN:1758-5082
ISSN (Online):1758-5090
Copyright Holders:Copyright © 2020 The Author(s)
First Published:First published in Biofabrication 12(4):045034
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering
301095UKRMP2 Acellular/Smart Materials 3D Architecture HubManuel Salmeron-SanchezMedical Research Council (MRC)MMRE_P75176 (MR/R015651/1ENG - Biomedical Engineering