Systematic reconstruction of the complete two-component sensorial network in Staphylococcus aureus

Rapun-Araiz, B., Haag, A.F. , De Cesare, V., Gil, C., Dorado-Morales, P., Penades, J.R. and Lasa, I. (2020) Systematic reconstruction of the complete two-component sensorial network in Staphylococcus aureus. mSystems, 5(4), e00511-20. (doi: 10.1128/mSystems.00511-20)

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Abstract

In bacteria, adaptation to changes in the environment is mainly controlled through two-component signal transduction systems (TCSs). Most bacteria contain dozens of TCSs, each of them responsible for sensing a different range of signals and controlling the expression of a repertoire of target genes (regulon). Over the years, identification of the regulon controlled by each individual TCS in different bacteria has been a recurrent question. However, limitations associated with the classical approaches used have left our knowledge far from complete. In this report, using a pioneering approach in which a strain devoid of the complete nonessential TCS network was systematically complemented with the constitutively active form of each response regulator, we have reconstituted the regulon of each TCS of S. aureus in the absence of interference between members of the family. Transcriptome sequencing (RNA-Seq) and proteomics allowed us to determine the size, complexity, and insulation of each regulon and to identify the genes regulated exclusively by one or many TCSs. This gain-of-function strategy provides the first description of the complete TCS regulon in a living cell, which we expect will be useful to understand the pathobiology of this important pathogen.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Penades, Prof Jose R and Haag, Dr Andreas
Authors: Rapun-Araiz, B., Haag, A.F., De Cesare, V., Gil, C., Dorado-Morales, P., Penades, J.R., and Lasa, I.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:mSystems
Publisher:American Society for Microbiology
ISSN:2379-5077
ISSN (Online):2379-5077
Published Online:18 August 2020
Copyright Holders:Copyright © 2020 Rapun-Araiz et al.
First Published:First published in mSystems 5(4): e00511-20
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172321DUT-SignalJose R PenadesEuropean Research Council (ERC)670932Institute of Infection, Immunity & Inflammation