Synchronous nanoscale topographic and chemical mapping by differential-confocal controlled Raman microscopy

Cui, H. , Wang, Y., Qiu, L., Li, S., Cooper, J. M. and Zhao, W. (2020) Synchronous nanoscale topographic and chemical mapping by differential-confocal controlled Raman microscopy. Photonics Research, 8(9), pp. 1441-1447. (doi: 10.1364/PRJ.394537)

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Abstract

Confocal Raman microscopy is currently used for label-free optical sensing and imaging within the biological, engineering, and physical sciences as well as in industry. However, currently these methods have limitations, including their low spatial resolution and poor focus stability, that restrict the breadth of new applications. This paper now introduces differential-confocal controlled Raman microscopy as a technique that fuses differential confocal microscopy and Raman spectroscopy, enabling the point-to-point collection of three-dimensional nanoscale topographic information with the simultaneous reconstruction of corresponding chemical information. The microscope collects the scattered Raman light together with the Rayleigh light, both as Rayleigh scattered and reflected light (these are normally filtered out in conventional confocal Raman systems). Inherent in the design of the instrument is a significant improvement in the axial focusing resolution of topographical features in the image (to ∼1 nm ), which, when coupled with super-resolution image restoration, gives a lateral resolution of 220 nm. By using differential confocal imaging for controlling the Raman imaging, the system presents a significant enhancement of the focusing and measurement accuracy, precision, and stability (with an antidrift capability), mitigating against both thermal and vibrational artefacts. We also demonstrate an improved scan speed, arising as a consequence of the nonaxial scanning mode.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Cui, Dr Han
Authors: Cui, H., Wang, Y., Qiu, L., Li, S., Cooper, J. M., and Zhao, W.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Photonics Research
Publisher:Optical Society of America
ISSN:2327-9125
ISSN (Online):2327-9125
Published Online:07 July 2020
Copyright Holders:Copyright © 2020 Chinese Laser Press
First Published:First published in Photonics Research 8(9): 1441-1447
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering