Howell, J., Hammarton, T. C. , Altmann, Y. and Jimenez, M. (2020) High-speed particle detection and tracking in microfluidic devices using event-based sensing. Lab on a Chip, 20(16), pp. 3024-3035. (doi: 10.1039/D0LC00556H) (PMID:32700715)
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Abstract
Visualising fluids and particles within channels is a key element of microfluidic work. Current imaging methods for particle image velocimetry often require expensive high-speed cameras with powerful illuminating sources, thus potentially limiting accessibility. This study explores for the first time the potential of an event-based camera for particle and fluid behaviour characterisation in a microfluidic system. Event-based cameras have the unique capacity to detect light intensity changes asynchronously and to record spatial and temporal information with low latency, low power and high dynamic range. Event-based cameras could consequently be relevant for detecting light intensity changes due to moving particles, chemical reactions or intake of fluorescent dyes by cells to mention a few. As a proof-of-principle, event-based sensing was tested in this work to detect 1 μm and 10 μm diameter particles flowing in a microfluidic channel for average fluid velocities of up to 1.54 m s−1. Importantly, experiments were performed by directly connecting the camera to a standard fluorescence microscope, only relying on the microscope arc lamp for illumination. We present a data processing strategy that allows particle detection and tracking in both bright-field and fluorescence imaging. Detection was achieved up to a fluid velocity of 1.54 m s−1 and tracking up to 0.4 m s−1 suggesting that event-based cameras could be a new paradigm shift in microscopic imaging.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Howell, Ms Jessie and Jimenez, Dr Melanie and Hammarton, Dr Tansy |
Authors: | Howell, J., Hammarton, T. C., Altmann, Y., and Jimenez, M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Lab on a Chip |
Publisher: | Royal Society of Chemistry |
ISSN: | 1473-0197 |
ISSN (Online): | 1473-0189 |
Published Online: | 17 July 2020 |
Copyright Holders: | Copyright © 2020 The Authors |
First Published: | First published in Lab on a Chip 20(16): 3024-3035 |
Publisher Policy: | Reproduced under a Creative Commons licence |
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