Human La is found at RNA polymerase III-transcribed genes in vivo

Fairley, J., Kantidakis, T., Kenneth, N., Intine, R., Maraia, R. and White, R. (2005) Human La is found at RNA polymerase III-transcribed genes in vivo. Proceedings of the National Academy of Sciences of the United States of America, 102(51), pp. 18350-18355. (doi: 10.1073/pnas.0506415102)

Abstract

The human La autoantigen can bind to nascent RNA transcripts and has also been postulated to act as an RNA polymerase III (pol III) transcription initiation and termination factor. Here, we show by chromatin immunoprecipitation (ChIP) that La is associated with pol III-transcribed genes in vivo. In contrast, the Ro autoantigen, which can also bind pol III transcripts, is not found at these genes. The putative pol III transcription factors NF1 and TFIIA are also not detected at class III genes. Binding of La remains when transcription is repressed at mitosis and does not correlate with the presence of polymerase at the gene. However, gene occupancy depends on the phosphorylation status of La, with the less prevalent, unphosphorylated form being found selectively on pol III templates.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: White, Prof Robert
• Authors: Fairley, J., Kantidakis, T., Kenneth, N., Intine, R., Maraia, R., and White, R.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Proceedings of the National Academy of Sciences of the United States of America
• Publisher: National Academy of Sciences
• ISSN: 0027-8424
• ISSN (Online): 1091-6490