Birchall, M., Idowu, B., Murison, P. , Jones, A., Burt, R., Ayling, S., Stokes, C., Pope, L. and Terenghi, G. (2004) Laryngeal abductor muscle reinnervation in a pig model. Acta Oto-Laryngologica, 124(7), pp. 839-846. (doi: 10.1080/00016480410022507) (PMID:15370570)
Full text not currently available from Enlighten.
Abstract
OBJECTIVE: To develop a large animal model for studies of laryngeal abductor reinnervation. MATERIAL AND METHODS: Six minipigs underwent unilateral anastomosis of the phrenic nerve-abductor branch of the recurrent laryngeal nerve (RLN). Polyhydroxybutyrate (PHB) conduits were used for repair. At each of 30, 60 and 120 days, 2 animals underwent video laryngeal endoscopy (VLE) and were then killed. VLE was also performed in the 120-day pair at 60 days. Nerve-conduit-nerve-muscle samples were fixed for light and immunofluorescence (pan-neurofilaments, S-100) microscopy. Laryngeal muscles were harvested (myosin heavy chain analysis). RESULTS: VLE showed recovery of abductor function in 1 animal at 60 days and in 1 at 120 days. Haematoxylin-eosin staining demonstrated a complex inflammatory response. Eosinophil recruitment was observed. Stepwise regeneration and reorganization of the distal nerve between 30 and 120 days was observed with pan-NF staining. The mean minimum diameter in the reinnervated posterior crico-arytenoids tended to increase for up to 120 days. CONCLUSIONS: Anastomosis of the phrenic nerve-abductor branch of the RLN with a PHB conduit in a pig can result in functional and histological recovery within 2-4 months and appears to at least sustain abductor muscle fibre morphology. Recovery occurs despite a complex inflammatory response, which may be an essential part of healing rather than inhibitory.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Murison, Professor Pamela |
Authors: | Birchall, M., Idowu, B., Murison, P., Jones, A., Burt, R., Ayling, S., Stokes, C., Pope, L., and Terenghi, G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Acta Oto-Laryngologica |
Publisher: | Taylor and Francis |
ISSN: | 0001-6489 |
ISSN (Online): | 1651-2251 |
University Staff: Request a correction | Enlighten Editors: Update this record