Novel biomarkers in cats with congestive heart failure due to primary cardiomyopathy

Liu, M., Eckersall, P. D. , Mrljak, V., Horvatić, A., Guillemin, N., Galan, A., Koster, L. and French, A. (2020) Novel biomarkers in cats with congestive heart failure due to primary cardiomyopathy. Journal of Proteomics, 226, 103896. (doi: 10.1016/j.jprot.2020.103896) (PMID:32652222)

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Abstract

The pathogenesis of feline cardiomyopathy and congestive heart failure (CHF) requires further understanding. In this study, we assessed serum proteome change in feline CHF, aiming to identify novel biomarker for both research and clinical use. The study comprised 15 cats in CHF, 5 cats in preclinical cardiomyopathy and 15 cats as healthy controls. Serum proteome profiles were obtained by tandem mass tag labelling followed by mass spectrometry. Protein concentrations in CHF cats were compared with healthy controls. Western blot was performed for proteomic validation. Correlations were assessed between the altered proteins in CHF and clinical variables in cats with cardiomyopathy to evaluate protein-cardiac association. Bioinformatic analysis was employed to identify pathophysiological pathways involved in feline CHF. Sixteen serum proteins were significantly different between CHF and healthy control cats (P < .05). These included serine protease inhibitors, apolipoproteins and other proteins associated with inflammation and coagulation. Clinical parameters from cats with cardiomyopathy significantly correlated with the altered proteins (P < .05). Bioinformatic analysis identified 13 most relevant functional profiles in feline CHF, which mostly associated with extracellular matrix organization and metabolism. Data are available via ProteomeXchange with identifier PXD017761.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Eckersall, Professor David and French, Professor Anne and Horvatic, Ms Anita and Liu, Mengmeng and Koster, Dr Liza
Creator Roles:
Liu, M.Conceptualization, Methodology, Investigation, Formal analysis, Writing – original draft, Visualization
Eckersall, P. D.Conceptualization, Methodology, Writing – review and editing, Supervision, Project administration
Horvatić, A.Investigation, Formal analysis, Data curation, Resources, Writing – review and editing
Koster, L.Resources, Writing – review and editing, Supervision, Funding acquisition
French, A.Conceptualization, Methodology, Resources, Writing – review and editing, Supervision, Project administration, Funding acquisition
Authors: Liu, M., Eckersall, P. D., Mrljak, V., Horvatić, A., Guillemin, N., Galan, A., Koster, L., and French, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Journal of Proteomics
Publisher:Elsevier
ISSN:1874-3919
ISSN (Online):1876-7737
Published Online:09 July 2020
Copyright Holders:Copyright © 2020 Elsevier B.V.
First Published:First published in Journal of Proteomics 226: 103896
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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