Synergy among exocyst and SNARE interactions identifies a functional hierarchy in secretion during vegetative growth

Larson, E. R., Ortmannová, J., Donald, N. A., Alvim, J., Blatt, M. R. and Žárský, V. (2020) Synergy among exocyst and SNARE interactions identifies a functional hierarchy in secretion during vegetative growth. Plant Cell, 32(9), pp. 2951-2963. (doi: 10.1105/tpc.20.00280) (PMID:32699172)

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Abstract

Vesicle exocytosis underpins signaling and development in plants and is vital for wall remodeling during cell expansion. Vesicle tethering and fusion are thought to occur sequentially, tethering mediated by the exocyst and fusion driven by assembly of SNARE protein complexes. Interactions between these two protein complexes are known, although insights into their functional consequences are largely unexplored. We now identify a clear hierarchy of interactions leading to secretion in Arabidopsis. Mating-based split-ubiquitin screens and in vivo FRET analyses showed that exocyst EXO70 subunits bind preferentially with cognate plasma membrane SNAREs, notably SYP121 and VAMP721. The exo70A1 mutant affected SNARE distributions and suppressed vesicle traffic like the dominant-negative SYP121deltaC, consistent with the epistasis of exo70A1 over the exo70A1syp121 double mutant. However, the exo70A1vamp721 mutant showed a strong synergistic suppression of growth. These data are best explained by a spatiotemporal hierarchy of exocyst recruitment with the R-SNARE to the plasma membrane, plausibly with the VAMP721 longin domain as a nexus for binding.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Blatt, Professor Michael and Alvim, Mr Jonas and Larson, Dr Emily and Donald, Miss Naomi
Authors: Larson, E. R., Ortmannová, J., Donald, N. A., Alvim, J., Blatt, M. R., and Žárský, V.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Cell
Publisher:American Society of Plant Biologists
ISSN:1040-4651
ISSN (Online):1532-298X
Published Online:22 June 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Plant Cell 32(9): 2951-2963
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172302Dissecting a new and vital checkpoint in SNARE recycling and plant growthMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/N006909/1MCSB - Plant Sciences
173107SAURs in stomatal regulation New Title: 15 NSFBIO SAUR regulation of stomatal apertureMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/P011586/1MCSB - Plant Sciences
172696Synthesis of Microcompartments in Plants for Enhanced Carbon FixationMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/N01832X/1MCSB - Plant Sciences
172831Exocyst and SNARE complex interactions in ArabidopsisMichael BlattThe Royal Society (ROYSOC)IE150953MCSB - Plant Sciences