Molina, S., Sanz, M. A., Madan, V., Ventoso, I., Castello, A. and Carrasco, L. (2007) Differential inhibition of cellular and Sindbis virus translation by brefeldin A. Virology, 363(2), pp. 430-436. (doi: 10.1016/j.virol.2007.02.001) (PMID:17360015)
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Abstract
Brefeldin A is a macrolide compound that interferes with the secretory pathway and also affects protein synthesis in mammalian cells. As a result, this antibiotic impedes the maturation of viral glycoproteins of enveloped viruses and viral genome replication in several virus species. In the present work, we show that translation of subgenomic mRNA from Sindbis virus, which in contrast to cellular translation is resistant to brefeldin A after prolonged treatment. The phosphorylation of eIF2α as a result of brefeldin A treatment correlates with the inhibition of cellular translation, while late viral protein synthesis is resistant to this phosphorylation. The effect of brefeldin A on Sindbis virus replication was also examined using a Sindbis virus replicon. Although brefeldin A delayed viral RNA synthesis, translation by non-replicative viral RNAs was not affected, reinforcing the idea that brefeldin A delays viral RNA replication, but does not directly affect Sindbis virus protein synthesis.
| Item Type: | Articles |
|---|---|
| Additional Information: | This study was supported by Grant (BFU2006-02182/BMC) from DGICYT and an Institutional Grant awarded to the Centro de Biología Molecular “Severo Ochoa” by the Fundación Ramón Areces. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Castello, Professor Alfredo |
| Authors: | Molina, S., Sanz, M. A., Madan, V., Ventoso, I., Castello, A., and Carrasco, L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Virology |
| Publisher: | Elsevier |
| ISSN: | 0042-6822 |
| ISSN (Online): | 1096-0341 |
| Published Online: | 23 March 2007 |
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