Impact of formate supplementation on body weight and plasma amino acids

Dhayade, S., Pietzke, M., Wiesheu, R., Tait-Mulder, J., Athineos, D., Sumpton, D., Coffelt, S. , Blyth, K. and Vazquez, A. (2020) Impact of formate supplementation on body weight and plasma amino acids. Nutrients, 12(8), 2181. (doi: 10.3390/nu12082181) (PMID:32708052) (PMCID:PMC7469024)

Abstract

Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM of sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology analyser. At the end of the study, blood and tissues were collected for metabolomics analysis and immune cell profiling. Formate supplementation had no significant physiological effect on male mice, except for a small decrease in body weight. Formate supplementation had no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice, however, the body weight and spleen wet weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of bifidobacteria, a probiotic bacterium, in the stools of female mice. We conclude that formate supplementation induces physiological changes in a gender-specific manner.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Wiesheu, Robert and Coffelt, Professor Seth and Tait-Mulder, Dr Jacqueline and Blyth, Professor Karen and Athineos, Mr Dimitris and Vazquez, Alexei and Sumpton, Mr David
• Authors: Dhayade, S., Pietzke, M., Wiesheu, R., Tait-Mulder, J., Athineos, D., Sumpton, D., Coffelt, S., Blyth, K., and Vazquez, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Research Centre: College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology
• Journal Name: Nutrients
• Publisher: MDPI
• ISSN: 2072-6643
• ISSN (Online): 2072-6643
• Copyright Holders: Copyright © 2020 by the authors.
• First Published: First published in Nutrients 12(8):2181
• Publisher Policy: Reproduced under a Creative Commons license