Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR

Wang, Y. et al. (2020) Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR. Microsystems and Nanoengineering, 6, 32. (doi: 10.1038/s41378-020-0146-2)

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Abstract

In this work, an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor (EGFR) by employing anti-EGFR aptamers as the bio-recognition element. The device used the concept of paper-folding, or origami, to serve as a valve between sample introduction and detection, so reducing sampling volumes and improving operation convenience. Amino-functionalized graphene (NH2-GO)/thionine (THI)/gold particle (AuNP) nanocomposites were used to modify the working electrode not only to generate the electrochemical signals, but also to provide an environment conducive to aptamer immobilization. Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode, thus resulting in a lower signal. The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL−1 (R2 = 0.989) and a detection limit of 5 pgmL−1 for EGFR. The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples, showing good agreement with the gold-standard enzyme-linked immunosorbent assay.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Yin, Professor Huabing and Reboud, Dr Julien and Yang, Dr Zhugen
Authors: Wang, Y., Sun, S., Luo, J., Xiong, Y., Ming, T., Liu, J., Ma, Y., Yan, S., Yang, Y., Yang, Z., Reboud, J., Yin, H., Cooper, J. M., and Cai, X.
College/School:College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Microsystems and Nanoengineering
Publisher:Nature Research
ISSN:2055-7434
ISSN (Online):2055-7434
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Microsystems and Nanoengineering 6: 32
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
302017EPSRC GCRF ISA 2017 - University of GlasgowLynne McCorristonEngineering and Physical Sciences Research Council (EPSRC)EP/R512813/1S&E - Business Development
168637Advanced Diagnostics using PhononicsJonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - Biomedical Engineering
300573Novel low cost diagnostic tools and their impact in AfricaJonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/R01437X/1ENG - Biomedical Engineering