The DNA sequence of chromosome I of an African trypanosome: gene content, chromosome organisation, recombination and polymorphism

Hall, N. et al. (2003) The DNA sequence of chromosome I of an African trypanosome: gene content, chromosome organisation, recombination and polymorphism. Nucleic Acids Research, 31(16), pp. 4864-4873. (doi:10.1093/nar/gkg674) (PMID:12907729) (PMCID:PMC169939)

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Abstract

The African trypanosome, Trypanosoma brucei, causes sleeping sickness in humans in sub‐Saharan Africa. Here we report the sequence and analysis of the 1.1 Mb chromosome I, which encodes approximately 400 predicted genes organised into directional clusters, of which more than 100 are located in the largest cluster of 250 kb. A 160‐kb region consists primarily of three gene families of unknown function, one of which contains a hotspot for retroelement insertion. We also identify five novel gene families. Indeed, almost 20% of predicted genes are members of families. In some cases, tandemly arrayed genes are 99–100% identical, suggesting an active process of amplification and gene conversion. One end of the chromosome consists of a putative bloodstream‐form variant surface glycoprotein (VSG) gene expression site that appears truncated and degenerate. The other chromosome end carries VSG and expression site‐associated genes and pseudogenes over 50 kb of subtelomeric sequence where, unusually, the telomere‐proximal VSG gene is oriented away from the telomere. Our analysis includes the cataloguing of minor genetic variations between the chromosome I homologues and an estimate of crossing‐over frequency during genetic exchange. Genetic polymorphisms are exceptionally rare in sequences located within and around the strand‐switches between several gene clusters.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:MacLeod, Professor Annette and Tait, Professor Andy and Turner, Professor Michael and Davies, Dr Robert
Authors: Hall, N., Berriman, M., Lennard, N. J., Harris, B. R., Hertz-Fowler, C., Bart-Delabesse, E., Gerrard, C. S., Atkin, R. J., Barron, A. J., Bowman, S., Bray-Allen, S. P., Bringaud, F., Clark, L. N., Corton, C. H., Cronin, A., Davies, R., Doggett, J., Fraser, A., Gruter, E., Hall, S., Harper, A. D., Kay, M. P., Leech, V., Mayes, R., Price, C., Quail, M. A., Rabbinowitsch, E., Reitter, C., Rutherford, K., Sasse, J., Sharp, S., Shownkeen, R., MacLeod, A., Taylor, S., Tweedie, A., Turner, C. M. R., Tait, A., Gull, K., Barrell, B., and Melville, S. E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN:0305-1048
ISSN (Online):1362-4962

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