Microdroplet based disposable sensor patch for detection of α-amylase in human blood serum

Bhattacharjee, M. , Middya, S., Escobedo, P. , Chaudhuri, J., Bandyopadhyay, D. and Dahiya, R. (2020) Microdroplet based disposable sensor patch for detection of α-amylase in human blood serum. Biosensors and Bioelectronics, 165, 112333. (doi: 10.1016/j.bios.2020.112333)

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Abstract

Concentration of α-amylase in human serum is a key indicator of various pancreatic ailments and an affordable point-of-care detection of this biomarker can benefit millions suffering from these diseases. In view of this situation, we report the development of a flexible patch-sensor, which simply requires a microdroplet of aqueous starch-FeSO4 solution to detect α-amylase in serum. The detection is achieved through the generation of mixing vortices (∼12 rpm) inside the droplet with the help of an imposed thermal gradient. Such vortices due to Marangoni and natural convections are found to be strongest at an optimal temperature difference of ∼18 °C – 23 °C across the droplet which in turn facilitate mixing and promote the specific starch-amylase enzymatic reaction. Subsequently, the large (∼80%) variation in the electrical resistance across the droplet is correlated to detect the level of the α-amylase in the analyte. Importantly, the sensor can detect even in the limits of 15–110 units/liter. Further, the sensitivity of flexible sensors is ∼8.6% higher than the non-flexible one. Interestingly, the sensitivity of the proposed sensor has been nearly three-times than the previously reported optical ones. The results of patch-sensor match very closely with the standard path-lab tests while detecting unknown level of amylase in serum. The prototype has shown significant potential to translate into an affordable device for the real-time detection and easy prognosis of pancreatic disorders.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Escobedo, Dr Pablo and Bhattacharjee, Mr Mitradip and Dahiya, Professor Ravinder
Creator Roles:
Bhattacharjee, M.Conceptualization, Investigation, Data curation, Methodology, Formal analysis, Writing – original draft
Escobedo, P.Investigation, Data curation, Methodology, Writing – original draft
Dahiya, R.Conceptualization, Formal analysis, Funding acquisition, Supervision, Writing – original draft
Authors: Bhattacharjee, M., Middya, S., Escobedo, P., Chaudhuri, J., Bandyopadhyay, D., and Dahiya, R.
College/School:College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Biosensors and Bioelectronics
Publisher:Elsevier
ISSN:0956-5663
ISSN (Online):1873-4235
Published Online:11 June 2020
Copyright Holders:Copyright © 2020 Elsevier B.V.
First Published:First published in Biosensors and Bioelectronics 165: 112333
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
301728Engineering Fellowships for Growth: Printed Tactile SKINRavinder DahiyaEngineering and Physical Sciences Research Council (EPSRC)EP/R029644/1ENG - Electronics & Nanoscale Engineering
170185Engineering Fellowships for Growth: Printed Tactile SKINRavinder DahiyaEngineering and Physical Sciences Research Council (EPSRC)EP/M002527/1ENG - Electronics & Nanoscale Engineering