Cell behavior within nanogrooved sandwich culture system

Bjørge, I. M., Salmeron-Sanchez, M. , Correia, C. R. and Mano, J. F. (2020) Cell behavior within nanogrooved sandwich culture system. Small, 16(31), 2001975. (doi: 10.1002/smll.202001975) (PMID:32603002)

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Abstract

Grooved topography and inherent cell contact guidance has shown promising results regarding cell proliferation, morphology, and lineage‐specific differentiation. Yet these approaches are limited to 2D applications. Sandwich‐culture conditions are developed to bridge the gap between 2D and 3D culture, enabling both ventral and dorsal cell surface stimulation. The effect of grooved surface topography is accessed on cell orientation and elongation in a highly controlled manner, with simultaneous and independent stimuli on two cell sides. Nanogrooved and non‐nanogrooved substrates are assembled into quasi‐3D systems with variable relative orientations. A plethora of sandwich‐culture conditions are created by seeding cells on lower, upper, or both substrates. Software image analysis demonstrates that F‐actin of cells acquires the orientation of the substrate on which cells are initially seeded, independently from the orientation of the second top substrate. Contrasting cell morphologies are observed, with a higher elongation for nanogrooved 2D substrates than nanogrooved sandwich‐culture conditions. Correlated with an increased pFAK activity and vinculin staining for sandwich‐culture conditions, these results point to an enhanced cell surface stimulation versus control conditions. The pivotal role of initial cell‐biomaterial contact on cellular alignment is highlighted, providing important insights for tissue engineering strategies aiming to guide cellular response through mechanotransduction approaches.

Item Type:Articles
Additional Information:I. M. Bjørge acknowledges financial support by the Portuguese Foundation for Science and Technology (FCT) with doctoral grant SFRH/BD/129224/2017. This work was supported by the European Research Council grant agreement for the project “ATLAS” (ERC-2014-ADG-669858) and the FCT project “CIRCUS” (PTDC/BTM-MAT/31064/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. Image acquisition was performed in the LiM facility of iBiMED, a node of PPBI (Portuguese Platform of BioImaging): POCI-01-0145-FEDER-022122.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel
Authors: Bjørge, I. M., Salmeron-Sanchez, M., Correia, C. R., and Mano, J. F.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Small
Publisher:Wiley
ISSN:1613-6810
ISSN (Online):1613-6829
Published Online:30 June 2020
Copyright Holders:Copyright © 2020 Wiley-VCH Verlag GmbH and Co. KGaA
First Published:First published in Small 16(31): 2001975
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering