The plasma proteome and the acute phase protein response in canine pyometra

Kuleš, J., Horvatić, A., Guillemin, N., Ferreira, R. F., Mischke, R., Mrljak, V., Chadwick, C. C. and Eckersall, P. D. (2020) The plasma proteome and the acute phase protein response in canine pyometra. Journal of Proteomics, 223, 103817. (doi: 10.1016/j.jprot.2020.103817) (PMID:32416315)

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Abstract

Canine pyometra is a common inflammatory disease of uterus in sexually mature bitches caused by secondary bacterial infection, leading to change in plasma proteins associated with the innate immune system. Proteomic investigation is increasingly being applied to canine diseases in order to identify and quantify significant changes in the plasma proteome. The aim of the study was to assess and quantify changes in plasma proteome profiles of healthy and pyometra affected bitches using a TMT-based high-resolution quantitative proteomic approach. As a result, 22 proteins were significantly down-regulated including transthyretin, antithrombin III, retinol-binding protein, vitamin D binding protein, paraoxonase 1, and kallikrein, while 16 were significantly up-regulated including haptoglobin light chain, alpha-1-acid glycoprotein, C-reactive protein precursor, and lipopolysaccharide-binding protein in dogs with pyometra. Pathway analysis indicated that acute inflammatory response, regulation of body fluid levels, protein activation cascade, the humoral immune response, and phagocytosis were affected in pyometra. Validation of biological relevance of the proteomic study was evident with significant increases in the concentrations of haptoglobin, C-reactive protein, alpha 1 acid glycoprotein, and ceruloplasmin by immunoassay. Pyometra in bitches was shown to stimulate an increase in host defence system proteins in response to inflammatory disease including the acute phase proteins.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Eckersall, Professor David and Horvatic, Ms Anita and Kules, Mrs Josipa
Authors: Kuleš, J., Horvatić, A., Guillemin, N., Ferreira, R. F., Mischke, R., Mrljak, V., Chadwick, C. C., and Eckersall, P. D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Proteomics
Publisher:Elsevier
ISSN:1874-3919
ISSN (Online):1876-7737
Published Online:13 May 2020
Copyright Holders:Copyright © 2020 Elsevier B.V.
First Published:First published in Journal of Proteomics 223: 103817
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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