Development of a high throughput and low cost model for the study of semi-dry biofilms

Amaeze, N., Akinbobola, A., Chukwuemeka, V., Abalkhaila, A., Ramage, G. , Kean, R., Staines, H., Williams, C. and Mackay, W. (2020) Development of a high throughput and low cost model for the study of semi-dry biofilms. Biofouling, 36(4), pp. 403-415. (doi: 10.1080/08927014.2020.1766030) (PMID:32441116)

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Abstract

The persistence of microorganisms as biofilms on dry surfaces resistant to the usual terminal cleaning methods may pose an additional risk of transmission of infections. In this study, the Centre for Disease Control (CDC) dry biofilm model (DBM) was adapted into a microtiter plate format (Model 1) and replicated to create a novel in vitro model that replicates conditions commonly encountered in the healthcare environment (Model 2). Biofilms of Staphylococcus aureus grown in the two models were comparable to the biofilms of the CDC DBM in terms of recovered log10 CFU well−1. Assessment of the antimicrobial tolerance of biofilms grown in the two models showed Model 2 a better model for biofilm formation. Confirmation of the biofilms’ phenotype with an extracellular matrix deficient S. aureus suggested stress tolerance through a non-matrix defined mechanism in microorganisms. This study highlights the importance of conditions maintained in bacterial growth as they affect biofilm phenotype and behaviour.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon and MacKay, Dr William and Kean, Mr Ryan and Williams, Dr Craig
Authors: Amaeze, N., Akinbobola, A., Chukwuemeka, V., Abalkhaila, A., Ramage, G., Kean, R., Staines, H., Williams, C., and Mackay, W.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Biofouling
Publisher:Taylor & Francis
ISSN:0892-7014
ISSN (Online):1029-2454
Published Online:22 May 2020
Copyright Holders:
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First Published:First published in Biofouling 36(4):403-415
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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