Acoustic erythrocytometer for mechanically probing cell viscoelasticity

Link, A. and Franke, T. (2020) Acoustic erythrocytometer for mechanically probing cell viscoelasticity. Lab on a Chip, 20(11), pp. 1991-1998. (doi: 10.1039/C9LC00999J) (PMID:32367091)

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We demonstrate an acoustic device to mechanically probe a population of red blood cells at the single cell level. The device operates by exciting a surface acoustic wave in a microfluidic channel creating a stationary acoustic wave field of nodes and antinodes. Erythrocytes are attracted to the nodes and are deformed. Using a stepwise increasing and periodically oscillating acoustic field we study the static and dynamic deformation of individual red blood cells one by one. We quantify the deformation by the Taylor deformation index D and relaxation times τ1 and τ2 that reveal both the viscous and elastic properties of the cells. The precision of the measurement allows us to distinguish between individual cells in the suspension and provides a quantitative viscoelastic fingerprint of the blood sample at single cell resolution. The method overcomes limitations of other techniques that provide averaged values and has the potential for high-throughput.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Link, Andreas and Franke, Professor Thomas
Authors: Link, A., and Franke, T.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN (Online):1473-0189
Published Online:21 April 2020
Copyright Holders:Copyright © 2020 The Royal Society of Chemistry
First Published:First published in Lab on a Chip 20(11): 1991-1998
Publisher Policy:Reproduced under a Creative Commons License

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