Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts

Mattos, A., Kusel, J., Pimenta, P. and Coelho, P. (2006) Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memorias Do Instituto Oswaldo Cruz, 101(Suppl1), pp. 283-287. (doi: 10.1590/s0074-02762006000900044) (PMID:17308783)

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Abstract

Praziquantel (PZQ) is effective against all the evolutive phases of Schistosoma mansoni. Infected Biomphalaria glabrata snails have their cercarial shedding interrupted when exposed to PZQ. Using primary in vitro transformed sporocysts, labeled with the probe Hoechst 33258 (indicator of membrane integrity), and lectin of Glycine max (specific for carbohydrate of N-acetylgalactosamine membrane), we evaluated the presence of lysosomes at this evolutive phase of S. mansoni, as well as the influence of PZQ on these acidic organelles and on the tegument of the sporocyst. Although the sporocyst remained alive, it was observed that there was a marked contraction of its musculature, and there occurred a change in the parasite's structure. Also, the acidic vesicles found in the sporocysts showed a larger delimited area after contact of the parasites with PZQ. Damages to the tegument was also observed, as show a well-marked labeling either with Hoechst 33258 or with lectin of Glycine max after contact of sporocysts with the drug. These results could partially explain the interruption/reduction mechanism of cercarial shedding in snails exposed to PZQ.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kusel, Dr John
Authors: Mattos, A., Kusel, J., Pimenta, P., and Coelho, P.
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Memorias Do Instituto Oswaldo Cruz
Publisher:Instituto Oswaldo Cruz
ISSN:0074-0276
ISSN (Online):1678-8060

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