Capsule endoscopy compatible fluorescence imager demonstrated using bowel cancer tumours

Melino, G. et al. (2020) Capsule endoscopy compatible fluorescence imager demonstrated using bowel cancer tumours. IEEE Sensors Journal, 20(17), pp. 9763-9771. (doi: 10.1109/JSEN.2020.2990293)

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Abstract

We demonstrate a proof of concept highly miniaturised fluorescence imager and its application to detecting cancer in resected human colon cancer tissues. Fluorescence imaging modalities have already been successfully implemented in traditional endoscopy. However, the procedure still causes discomfort and requires sedation. Wireless fluorescence capsule endoscopy has the potential to improve diagnostic accuracy with less inconvenience for patients. In this paper we present a 5 mm x 6 mm x 5 mm optical block that is small enough to integrate into a capsule endoscope. The block integrates ultrathin filters for optical isolation and was successfully integrated with a sensitive CMOS SPAD array to detect green fluorescence from Flavin Adenine Dinucleotide (FAD), which is an endogenous fluorophore responsible for autofluorescence in human tissues, and fluorescence from the cancer selective molecular probe ProteoGREENTM-gGlu used to label colorectal cancer cells. In vitro studies were validated using a commercial ModulusTM Microplate reader. The potential use of the device in capsule endoscopy was further validated by imaging healthy and malignant resected human tissues from the colon to detect changes in autofluorescence signal that are crucial for cancer diagnosis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Al-Rawhani, Dr Mohammed and Escorcia Carranza, Dr Ivonne and Accarino, Claudio and MELINO, GIANLUCA and Grant, Dr James and Cumming, Professor David and Riehle, Dr Mathis and Annese, Valerio and Beeley, Dr James
Authors: Melino, G., Accarino, C., Riehle, M., Potter, M., Fineron, P., Annese, V. F., Grant, J. P., Al-Rawhani, M., Beeley, J., Escorcia Carranza, I., and Cumming, D. R.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
College of Science and Engineering > School of Engineering > Systems Power and Energy
Journal Name:IEEE Sensors Journal
Publisher:IEEE
ISSN:1530-437X
ISSN (Online):1558-1748
Published Online:24 April 2020
Copyright Holders:Copyright © 2020 IEEE
First Published:First published in IEEE Sensors Journal 20(17): 9763-9771
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
169106Sonopill: minimally invasive gastrointestinal diagnosis and therapyDavid CummingEngineering and Physical Sciences Research Council (EPSRC)EP/K034537/1ENG - Electronics & Nanoscale Engineering