A global non-coding RNA system modulates fission yeast protein levels in response to stress

Leong, H. S., Dawson, K., Wirth, C., Li, Y., Connolly, Y., Smith, D. L., Wilkinson, C. R. M. and Miller, C. J. (2014) A global non-coding RNA system modulates fission yeast protein levels in response to stress. Nature Communications, 5, 3947. (doi: 10.1038/ncomms4947) (PMID:24853205) (PMCID:PMC4050258)

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Abstract

Non-coding RNAs (ncRNAs) are frequent and prevalent across the taxa. Although individual non-coding loci have been assigned a function, most are uncharacterized. Their global biological significance is unproven and remains controversial. Here we investigate the role played by ncRNAs in the stress response of Schizosaccharomyces pombe. We integrate global proteomics and RNA sequencing data to identify a systematic programme in which elevated antisense RNA arising both from ncRNAs and from 3′-overlapping convergent gene pairs is directly associated with substantial reductions in protein levels throughout the genome. We describe an extensive array of ncRNAs with trans associations that have the potential to influence multiple pathways. Deletion of one such locus reduces levels of atf1, a transcription factor downstream of the stress-activated mitogen-activated protein kinase (MAPK) pathway, and alters sensitivity to oxidative stress. These non-coding transcripts therefore regulate specific stress responses, adding unanticipated information-processing capacity to the MAPK signalling system.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Miller, Professor Crispin
Authors: Leong, H. S., Dawson, K., Wirth, C., Li, Y., Connolly, Y., Smith, D. L., Wilkinson, C. R. M., and Miller, C. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Nature Communications
Publisher:Nature Research
ISSN:2041-1723
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2014 Macmillan Publishers Limited
First Published:First published in Nature Communications 5:3947
Publisher Policy:Reproduced under a Creative Commons Licence

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