Dietary fibres differentially impact on the production of phenolic acids from rutin in an in vitro fermentation model of the human gut microbiota

Havlik, J. , Marinello, V., Gardyne, A., Hou, M., Mullen, W. , Morrison, D. J. , Preston, T. , Combet, E. and Edwards, C. A. (2020) Dietary fibres differentially impact on the production of phenolic acids from rutin in an in vitro fermentation model of the human gut microbiota. Nutrients, 12(6), 1577. (doi: 10.3390/nu12061577)

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Abstract

Polyphenols are often ingested alongside dietary fibres. They are both catabolised by, and may influence, the intestinal microbiota; yet, interactions between them and the impact on their resultant microbial products are poorly understood. Dietary fibres (inulin, pectin, psyllium, pyrodextrin, wheat bran, cellulose—three doses) were fermented in vitro with human faeces (n = 10) with and without rutin (20 µg/mL), a common dietary flavonol glycoside. Twenty-eight phenolic metabolites and short chain fatty acids (SCFA) were measured over 24 h. Several phenolic metabolites were produced during fibre fermentation, without rutin. With rutin, 3,4-dihydroxyphenylacetic acid (3,4diOHPAA), 3-hydroxyphenylacetic acid (3OHPAA), 3-(3 hydroxyphenyl)propionic acid (3OHPPA) and 3-(3,4-dihydroxyphenyl)propionic acid (3,4diOHPPA; DOPAC) were produced, with 3,4diOHPAA the most abundant, confirmed by fermentation of 13C labelled quercetin. The addition of inulin, wheat bran or pyrodextrin increased 3,4diOHPAA 2 2.5-fold over 24 h (p < 0.05). Rutin affected SCFA production, but this depended on fibre, fibre concentration and timepoint. With inulin, rutin increased pH at 6 h from 4.9 to 5.6 (p = 0.01) but increased propionic, butyric and isovaleric acid (1.9, 1.6 and 5-fold, p < 0.05 at 24 h). Interactions between fibre and phenolics modify production of phenolic acids and SCFA and may be key in enhancing health benefits.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mullen, Dr Bill and Edwards, Professor Christine and Preston, Professor Tom and Morrison, Dr Douglas and Combet Aspray, Dr Emilie and Hou, Miss Min and Havlik, Dr Jaroslav
Creator Roles:
Edwards, C. A.Conceptualization, Methodology, Validation, Resources, Data curation, Writing – review and editing, Supervision, Project administration, Funding acquisition
Combet, E.Conceptualization, Methodology, Writing – review and editing, Supervision
Havlik, J.Methodology, Software, Investigation, Writing – original draft, Visualization
Morrison, D. J.Methodology, Writing – review and editing, Supervision
Preston, T.Formal analysis, Supervision
Mullen, W.Formal analysis, Supervision
Authors: Havlik, J., Marinello, V., Gardyne, A., Hou, M., Mullen, W., Morrison, D. J., Preston, T., Combet, E., and Edwards, C. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Nutrients
Publisher:MDPI
ISSN:2072-6643
ISSN (Online):2072-6643
Published Online:28 May 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Nutrients 12(6): 1577
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190921Manipulating the activity of the gut microbiota with fermentable carbohydrates to maximise the bioavailability of bioactive phenolic acids for healthChristine EdwardsBiotechnology and Biological Sciences Research Council (BBSRC)BB/M027724/1Med - Human Nutrition