Measurement of absorption in scattering media using objective laser speckle: application to blood oximetry

Carles, G. , Brewer, L. and Harvey, A. R. (2020) Measurement of absorption in scattering media using objective laser speckle: application to blood oximetry. Optics Express, 28(4), pp. 5119-5133. (doi: 10.1364/OE.383318) (PMID:32121739)

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Abstract

Multi-spectral imaging enables non-invasive sensing of chemical concentrations in biological tissue based on measurement of optical absorption, but invariably in the presence of high levels of scatter. Absorption is normally inferred from measurement of contrast of biological features, such as the vasculature, and so accuracy is degraded by the poorly characterized modulation-transfer function of the imaging optics and overlying tissue. We report how experimental characterization of the spectral variation of the tissue point-spread function and associated objective speckle pattern can be used to characterize the absorption spectrum and chromophore concentration, with a particular emphasis on determination of the ratio of oxygenated to deoxygenated hemoglobin within blood. Absorption measurements are determined purely by the geometry of the experiment, without degradation due to optical aberrations and associated light scatter. The technique offers enhanced and low-cost determination of in vitro or in vivo chromophore characterizations, including blood-gas analysis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Carles Santacana, Dr Guillem and Brewer, Dr Laurence and Harvey, Professor Andy
Authors: Carles, G., Brewer, L., and Harvey, A. R.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
ISSN (Online):1094-4087
Published Online:10 February 2020
Copyright Holders:Copyright © 2020 Optical Society of America
First Published:First published in Optics Express 28(4): 5119-5133
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173569Seeing within extended volumes and 3D structures: 3D imaging and applicationsGuillem Carles SantacanaLeverhulme Trust (LEVERHUL)ECF-2016-757P&S - Physics & Astronomy
162767Scottish Universities Physics Alliance (SUPA): A Partnership for ExcellenceMartin HendryScottish Funding Council (SFC)HR09013P&S - Administration