Growth of Piscirickettsia salmonis to high Titers in insect tissue culture cells

Birkbeck, T.H., Griffen, A. A., Reid, H. I., Laidler, L. A. and Wadsworth, S. (2004) Growth of Piscirickettsia salmonis to high Titers in insect tissue culture cells. Infection and Immunity, 72(6), pp. 3693-3694. (doi:10.1128/IAI.72.6.3693-3694.2004) (PMID:15155687) (PMCID:PMC415715)

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Abstract

Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24°C.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gadegaard, Dr Helen and Birkbeck, Professor Harry
Authors: Birkbeck, T.H., Griffen, A. A., Reid, H. I., Laidler, L. A., and Wadsworth, S.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Infection and Immunity
Publisher:American Society for Microbiology
ISSN:0019-9567
ISSN (Online):1098-5522

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
288621Ecology of the fish-pathogenic rickettsiaHarry BirkbeckNatural Environment Research Council (NERC)NER/C/S/2000/00SCHOOL OF LIFE SCIENCES