Twin-Airy point-spread function for extended-volume particle localization

Zhou, Y. , Zammit, P., Zickus, V., Taylor, J. M. and Harvey, A. R. (2020) Twin-Airy point-spread function for extended-volume particle localization. Physical Review Letters, 124(19), 198104. (doi: 10.1103/PhysRevLett.124.198104) (PMID:32469536)

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Abstract

The localization of point sources in optical microscopy enables nm-precision imaging of single-molecules and biological dynamics. We report a new method of localization microscopy using twin Airy beams that yields precise 3D localization with the key advantages of extended depth range, higher optical throughput, and potential for imaging higher emitter densities than are possible using other techniques. A precision of better than 30 nm was achieved over a depth range in excess of 7μm using a 60×, 1.4 NA objective. An illustrative application to extended-depth-range blood-flow imaging in a live zebrafish is also demonstrated.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Zammit, Mr Paul and Taylor, Dr Jonathan and Harvey, Professor Andy and Zhou, Mr Yongzhuang and Zickus, Dr Vytautas
Authors: Zhou, Y., Zammit, P., Zickus, V., Taylor, J. M., and Harvey, A. R.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Physical Review Letters
Publisher:American Physical Society
ISSN:0031-9007
ISSN (Online):1079-7114
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Physical Review Letters 124(19):198104
Publisher Policy:Reproduced under a Creative Commons licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190490Engineering and Physical Sciences Doctoral Training Grant 2012-16Mary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/K503058/1Research and Innovation Services
190790EPSRC DTG 2014Mary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/M506539/1Research and Innovation Services