Plasma polymerised nanoscale coatings of controlled thickness for efficient solid-phase presentation of growth factors

Alba, A., Jayawarna, V., Childs, P. G., Dalby, M. J. and Salmeron-Sanchez, M. (2020) Plasma polymerised nanoscale coatings of controlled thickness for efficient solid-phase presentation of growth factors. Materials Science and Engineering C: Biomimetic and Supramolecular Systems, (doi: 10.1016/j.msec.2020.110966) (Early Online Publication)

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Abstract

The engineering of biomaterial surfaces and scaffolds for specific biomedical and clinical application is of growing interest. Certain functionalised surfaces can capture and deliver bioactive molecules, such as growth factors (GF), enhancing the clinical efficacy of such systems. With a custom-made plasma polymerisation reactor described here we have developed bioactive polymer coatings based on poly(ethyl acrylate) (PEA). This remarkable polymer unfolds fibronectin (FN) upon adsorption to allow the GF binding region of FN to sequester and present GFs with high efficiency. We systematically evaluate process conditions and their impact on plasma polymerised PEA coatings and we characterise the effect of plasma power and deposition time on thickness, wettability and chemical composition of the coatings. We demonstrate that functional substrate roughness can be maintained after deposition of the polymer coatings. Importantly, we show that coatings deposited at different conditions all maintain a similar or better bioactivity than spin coated PEA references. We show that in PEA plasma polymerised coatings fibronectin assembles into nanonetworks with high availability of integrin and GF binding regions that sequester bone morphogenetic protein-2 (BMP-2). We also report similar mesenchymal stem cell adhesion behaviour, as characterised by focal adhesions, and differentiation potential on BMP-2 coated surfaces, regardless of plasma deposition conditions. This is a potent and versatile technology that can help facilitate the use of GFs in clinical applications.

Item Type:Articles
Status:Early Online Publication
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Jayawarna, Dr Vineetha and Childs, Dr Peter and Alba Perez, Dr Andres
Creator Roles:
Alba, A.Conceptualization, Methodology, Data curation, Writing – original draft
Jayawarna, V.Conceptualization, Methodology, Data curation, Writing – original draft
Childs, P. G.Conceptualization, Methodology, Writing – review and editing
Dalby, M. J.Conceptualization, Supervision, Writing – review and editing
Salmeron-Sanchez, M.Conceptualization, Supervision, Funding acquisition, Writing – original draft
Authors: Alba, A., Jayawarna, V., Childs, P. G., Dalby, M. J., and Salmeron-Sanchez, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Materials Science and Engineering C: Biomimetic and Supramolecular Systems
Publisher:Elsevier
ISSN:0928-4931
ISSN (Online):1873-0191
Published Online:13 April 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Materials Science and Engineering C: Biomimetic and Supramolecular Systems 2020
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering
301095UKRMP2 Acellular/Smart Materials 3D Architecture HubManuel Salmeron-SanchezMedical Research Council (MRC)MR/R015651/1ENG - Biomedical Engineering