Trypanosoma brucei Polo-like kinase is essential for basal body duplication, kDNA segregation and cytokinesis

Hammarton, T. C. , Kramer, S., Tetley, L., Boshart, M. and Mottram, J. C. (2007) Trypanosoma brucei Polo-like kinase is essential for basal body duplication, kDNA segregation and cytokinesis. Molecular Microbiology, 65(5), pp. 1229-1248. (doi:10.1111/j.1365-2958.2007.05866.x)

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Publisher's URL: http://dx.doi.org/10.1111/j.1365-2958.2007.05866.x

Abstract

Polo-like kinases (PLKs) are conserved eukaryotic cell cycle regulators, which play multiple roles, particularly during mitosis. The function of Trypanosoma brucei PLK was investigated in procyclic and bloodstream-form parasites. In procyclic trypanosomes, RNA interference (RNAi) of PLK, or overexpression of TY1-epitope-tagged PLK (PLKty), but not overexpression of a kinase-dead variant, resulted in the accumulation of cells that had divided their nucleus but not their kinetoplast (2N1K cells). Analysis of basal bodies and flagella in these cells suggested the defect in kinetoplast division arose because of an inhibition of basal body duplication, which occurred when PLK expression levels were altered. Additionally, a defect in kDNA replication was observed in the 2N1K cells. However, the 2N1K cells obtained by each approach were not equivalent. Following PLK depletion, the single kinetoplast was predominantly located between the two divided nuclei, while in cells overexpressing PLKty, the kinetoplast was mainly found at the posterior end of the cell, suggesting a role for PLK kinase activity in basal body and kinetoplast migration. PLK RNAi in bloodstream trypanosomes also delayed kinetoplast division, and was further observed to inhibit furrow ingression during cytokinesis. Notably, no additional roles were detected for trypanosome PLK in mitosis, setting this protein kinase apart from its counterparts in other eukaryotes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tetley, Dr Laurence and Hammarton, Dr Tansy and Mottram, Professor Jeremy
Authors: Hammarton, T. C., Kramer, S., Tetley, L., Boshart, M., and Mottram, J. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular Microbiology
Publisher:Wiley-Blackwell Publishing Ltd.
ISSN:0950-382X
ISSN (Online):1365-2958
Published Online:27 July 2007
Copyright Holders:Copyright © 2007 Blackwell Publishing Ltd
First Published:First published in Molecular Microbiology 65(5):1229-1248
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
403591Regulation of cytokinesis in trypanosoma bruceiTansy HammartonMedical Research Council (MRC)G120/1001Infection Immunity and Inflammation Life Sciences