Toxoplasma gondii: further studies on the subpellicular network

Lemgruber, L. , Kloetzel, J. A., de Souza, W. and Vommaro, R. C. (2009) Toxoplasma gondii: further studies on the subpellicular network. Memorias Do Instituto Oswaldo Cruz, 104(5), pp. 706-709. (doi: 10.1590/S0074-02762009000500007) (PMID:19820829)

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Abstract

The association of the pellicle with cytoskeletal elements in Toxoplasma gondii allows this parasite to maintain its mechanical integrity and makes possible its gliding motility and cell invasion. The inner membrane complex (IMC) resembles the flattened membrane sacs observed in free-living protozoa and these sacs have been found to associate with cytoskeletal proteins such as articulins. We used immunofluorescence microscopy to characterise the presence and distribution of plateins, a sub-family of articulins, in T. gondii tachyzoites. A dispersed labelling of the whole protozoan body was observed. Electron microscopy of detergent-extracted cells revealed the presence of a network of 10 nm filaments distributed throughout the parasite. These filaments were labelled with anti-platein antibodies. Screening the sequenced T. gondii genome, we obtained the sequence of an IMC predicted protein with 25% identity and 42% similarity to the platein isoform alpha 1 present in Euplotes aediculatus, but with 42% identity and 55% similarity to that found in Euglena gracilis, suggesting strong resemblance to articulins.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lemgruber Soares, Dr Leandro
Authors: Lemgruber, L., Kloetzel, J. A., de Souza, W., and Vommaro, R. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Memorias Do Instituto Oswaldo Cruz
Publisher:Instituto Oswaldo Cruz
ISSN:0074-0276
ISSN (Online):1678-8060
Copyright Holders:Copyright © 2009 The Authors
First Published:First published in Memorias Do Instituto Oswaldo Cruz 104(5): 706-709
Publisher Policy:Reproduced under a Creative Commons License

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