Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion

Hanssen, E. et al. (2013) Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion. Cellular Microbiology, 15(9), pp. 1457-1472. (doi: 10.1111/cmi.12132) (PMID:23461734)

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Abstract

Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria disease. To date, efforts to understand cellular events underpinning entry have been limited to insights from non‐human parasites, with no studies at sub‐micrometer resolution undertaken using the most virulent human malaria parasite, P lasmodium falciparum . This leaves our understanding of the dynamics of merozoite sub‐cellular compartments during infectionincomplete, in particular that of the secretory organelles. Using advances in P . falciparum merozoite isolation and new imaging techniques we present a three‐dimensional study of invasion using electron microscopy, cryo‐electron tomography and cryo‐X‐ray tomography. We describe the core architectural features of invasion and identify fusion between rhoptries at the commencement of invasion as a hitherto overlooked event that likely provides a critical step that initiates entry. Given the centrality of merozoite organelle proteins to vaccine development, these insights provide a mechanistic framework to understand therapeutic strategies targeted towards the cellular events of invasion.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lemgruber Soares, Dr Leandro
Authors: Hanssen, E., Dekiwadia, C., Riglar, D. T., Rug, M., Lemgruber Soares, L., Cowman, A. F., Cyrklaff, M., Kudryashev, M., Frischknecht, F., Baum, J., and Ralph, S. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cellular Microbiology
Publisher:John Wiley & Sons, Ltd.
ISSN:1462-5814
ISSN (Online):1462-5822
Published Online:22 March 2013

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