Nanoscopic localization of surface-exposed antigens of Borrelia burgdorferi

Lemgruber Soares, L. , Sant'Anna, C., Griffiths, C., abud, y., Mhlanga, M., Wallich, R. and Frischknecht, F. (2015) Nanoscopic localization of surface-exposed antigens of Borrelia burgdorferi. Microscopy and Microanalysis, 21(3), pp. 680-688. (doi: 10.1017/S1431927615000318) (PMID:25739645)

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Abstract

Borrelia burgdorferi sensu lato, the causative agent of Lyme disease, is transmitted to humans through the bite of infected Ixodes spp. ticks. Successful infection of vertebrate hosts necessitates sophisticated means of the pathogen to escape the vertebrates’ immune system. One strategy employed by Lyme disease spirochetes to evade adaptive immunity involves a highly coordinated regulation of the expression of outer surface proteins that is vital for infection, dissemination, and persistence. Here we characterized the expression pattern of bacterial surface antigens using different microscopy techniques, from fluorescent wide field to super-resolution and immunogold-scanning electron microscopy. A fluorescent strain of B. burgdorferi spirochetes was labeled with monoclonal antibodies directed against various bacterial surface antigens. Our results indicate that OspA is more evenly distributed over the surface than OspB and OspC that were present as punctate areas.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:abud, yuri and Lemgruber Soares, Dr Leandro
Authors: Lemgruber Soares, L., Sant'Anna, C., Griffiths, C., abud, y., Mhlanga, M., Wallich, R., and Frischknecht, F.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Microscopy and Microanalysis
Publisher:Cambridge University Press
ISSN:1431-9276
ISSN (Online):1435-8115
Published Online:05 March 2015

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