Overexpression of the selective autophagy cargo receptor NBR1 modifies plant response to sulfur deficit

Tarnowski, L., Collados Rodriguez, M. , Brzywczy, J., Cysewski, D., Wawrzynska, A. and Sirko, A. (2020) Overexpression of the selective autophagy cargo receptor NBR1 modifies plant response to sulfur deficit. Cells, 9(3), e669. (doi: 10.3390/cells9030669) (PMID:32164165) (PMCID:PMC7140714)

Abstract

Plants exposed to sulfur deficit elevate the transcription of NBR1 what might reflect an increased demand for NBR1 in such conditions. Therefore, we investigated the role of this selective autophagy cargo receptor in plant response to sulfur deficit (-S). Transcriptome analysis of the wild type and NBR1 overexpressing plants pointed out differences in gene expression in response to -S. Our attention focused particularly on the genes upregulated by -S in roots of both lines because of significant overrepresentation of cytoplasmic ribosomal gene family. Moreover, we noticed overrepresentation of the same family in the set of proteins co-purifying with NBR1 in -S. One of these ribosomal proteins, RPS6 was chosen for verification of its direct interaction with NBR1 and proven to bind outside the NBR1 ubiquitin binding domains. The biological significance of this novel interaction and the postulated role of NBR1 in ribosomes remodeling in response to starvation remain to be further investigated. Interestingly, NBR1 overexpressing seedlings have significantly shorter roots than wild type when grown in nutrient deficient conditions in the presence of TOR kinase inhibitors. This phenotype probably results from excessive autophagy induction by the additive effect of NBR1 overexpression, starvation, and TOR inhibition.

• Item Type: Articles
• Additional Information: Funding: This research was funded by National Science Centre grant No. 2014/15/B/NZ3/04854, and MCR was supported by MSCA‐ITN, BIONUT. Polish National Agency for Academic Exchange (NAWA) is acknowledged for supporting bilateral French‐Polish collaboration (Polonium project No PPN/BIL/2018/1/00182/U/00003).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Collados Rodriguez, Dr Milagros

Creator Roles

Collados Rodriguez, M.: Investigation

• Authors: Tarnowski, L., Collados Rodriguez, M., Brzywczy, J., Cysewski, D., Wawrzynska, A., and Sirko, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Cells
• Publisher: MDPI
• ISSN: 2073-4409
• ISSN (Online): 2073-4409
• Copyright Holders: Copyright © 2020 by the authors
• First Published: First published in Cells 9(3):e669
• Publisher Policy: Reproduced under a Creative Commons license