Precise 3D particle localization over large axial ranges using secondary astigmatism

Zhou, Y. and Carles, G. (2020) Precise 3D particle localization over large axial ranges using secondary astigmatism. Optics Letters, 45(8), pp. 2466-2469. (doi: 10.1364/OL.388695) (PMID:32287260)

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We propose an analytical pupil phase function employing cropped secondary astigmatism for extended-depth nanoscale 3D-localization microscopy. The function provides high localization precision in all three dimensions, which can be maintained over extended axial ranges, customizable up to two orders of magnitude relative to the conventional, diffraction-limited imaging. This enables, for example, capturing nanoscale dynamics within a whole cell. The flexibility and simplicity in the implementation of the proposed phase function make its adoption in localization-based microscopy attractive. We demonstrate and validate its application to real-time imaging of 3D fluid flow over a depth of 40 µm with a numerical aperture of 0.8.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Carles Santacana, Dr Guillem and Zhou, Mr Yongzhuang
Authors: Zhou, Y., and Carles, G.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Letters
Publisher:Optical Society of America
ISSN (Online):1539-4794
Copyright Holders:Copyright © 2020 Optical Society of America
First Published:First published in Optics Letters 45(8):2466-2469
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173569Seeing within extended volumes and 3D structures: 3D imaging and applicationsGuillem Carles SantacanaLeverhulme Trust (LEVERHUL)ECF-2016-757P&S - Physics & Astronomy
171888University of Glasgow Experimental Equipment ProposalAndrew HarveyEngineering and Physical Sciences Research Council (EPSRC)EP/M028135/1P&S - Physics & Astronomy