Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis

Nakagawa, R., Mason, S.M. and Michie, A.M. (2006) Determining the role of specific signaling molecules during lymphocyte development in vivo: instant transgenesis. Nature Protocols, 1(3), pp. 1185-1193. (doi: 10.1038/nprot.2006.178)

Abstract

A common method of determining the role of specific signaling molecules during lymphocyte development is to generate a transgenic mouse. This procedure, while informative, is time consuming, expensive and ultimately does not guarantee a defined answer. Here we present a protocol in which the in vivo effects of a gene of interest on both B and T lymphocyte development may be determined simultaneously in a relatively short time period. This is achieved by introducing a defined gene, such as a wild-type or mutated signaling molecule, into a lymphoid progenitor population by retroviral infection. The retrovirus generates a bicistronic message encoding the gene of interest and GFP, thus enabling identification of retrovirally transduced cells in subsequent lymphocyte lineages. The cells are then introduced into mice deficient for recombinase activating gene 1 (Rag(-/-) mice), thus allowing the development of donor-derived B and T lymphocytes in vivo. Using this technique, results can be obtained within 3-8 weeks.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Michie, Professor Alison
• Authors: Nakagawa, R., Mason, S.M., and Michie, A.M.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Nature Protocols
• ISSN: 1754-2189