Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila

Pondeville, E., Puchot, N., Parvy, J.-P., Carissimo, G., Poidevin, M., Waterhouse, R. M., Marois, E. and Bourgouin, C. (2020) Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila. Insect Biochemistry and Molecular Biology, 120, 103339. (doi: 10.1016/j.ibmb.2020.103339) (PMID:32105779) (PMCID:PMC7181189)

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Abstract

Hemocytes, the immune cells in mosquitoes, participate in immune defenses against pathogens including malaria parasites. Mosquito hemocytes can also be infected by arthropod-borne viruses but the pro- or anti-viral nature of this interaction is unknown. Although there has been progress on hemocyte characterization during pathogen infection in mosquitoes, the specific contribution of hemocytes to immune responses and the hemocyte-specific functions of immune genes and pathways remain unresolved due to the lack of genetic tools to manipulate gene expression in these cells specifically. Here, we used the Gal4-UAS system to characterize the activity of the Drosophila hemocyte-specific hemolectin promoter in the adults of Anopheles gambiae, the malaria mosquito. We established an hml-Gal4 driver line that we further crossed to a fluorescent UAS responder line, and examined the expression pattern in the adult progeny driven by the hml promoter. We show that the hml regulatory region drives hemocyte-specific transgene expression in a subset of hemocytes, and that transgene expression is triggered after a blood meal. The hml promoter drives transgene expression in differentiating prohemocytes as well as in differentiated granulocytes. Analysis of different immune markers in hemocytes in which the hml promoter drives transgene expression revealed that this regulatory region could be used to study phagocytosis as well as melanization. Finally, the hml promoter drives transgene expression in hemocytes in which o’nyong-nyong virus replicates. Altogether, the Drosophila hml promoter constitutes a good tool to drive transgene expression in hemocyte only and to analyze the function of these cells and the genes they express during pathogen infection in Anopheles gambiae.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Pondeville, Dr Emilie and Parvy, Dr Jean-Philippe
Authors: Pondeville, E., Puchot, N., Parvy, J.-P., Carissimo, G., Poidevin, M., Waterhouse, R. M., Marois, E., and Bourgouin, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Insect Biochemistry and Molecular Biology
Publisher:Elsevier
ISSN:0965-1748
ISSN (Online):1879-0240
Published Online:24 February 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Insect Biochemistry and Molecular Biology 120: 103339
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656551Arbovirus interactions with arthropod hostsAlain KohlMedical Research Council (MRC)MC_UU_12014/8MVLS III - CENTRE FOR VIRUS RESEARCH