Analysis of lung stromal expression of the atypical chemokine receptor ACKR2 reveals unanticipated expression in blood endothelial cells

Hansell, C. A.H., Love, S., Pingen, M., Wilson, G. J. , Macleod, M. and Graham, G. (2020) Analysis of lung stromal expression of the atypical chemokine receptor ACKR2 reveals unanticipated expression in blood endothelial cells. European Journal of Immunology, 50(5), pp. 666-675. (doi: 10.1002/eji.201948374) (PMID:32114694)

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Abstract

Analysis of chemokine receptor, and atypical chemokine receptor, expression is frequently hampered by the lack of availability of high‐quality antibodies and the species specificity of those that are available. We have previously described methodology utilizing Alexa‐Fluor‐labeled chemokine ligands as versatile reagents to detect receptor expression. Previously this has been limited to hematopoietic cells and methodology for assessing expression of receptors on stromal cells has been lacking. Among chemokine receptors, the ones most frequently expressed on stromal cells belong to the atypical chemokine receptor subfamily. These receptors do not signal in the classic sense in response to ligand but scavenge their ligands and degrade them and thus sculpt in vivo chemokine gradients. Here, we demonstrate the ability to use either intratracheal or intravenous, Alexa‐Fluor‐labeled chemokine administration to detect stromal cell populations expressing the atypical chemokine receptor ACKR2. Using this methodology, we demonstrate, for the first time, expression of ACKR2 on blood endothelial cells. This observation sets the lung aside from other tissues in which ACKR2 is exclusively expressed on lymphatic endothelial cells and suggest unique roles for ACKR2 in the pulmonary environment.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Pingen, Dr Marieke and Hansell, Dr Chris and Wilson, Dr Gillian and Graham, Professor Gerard and Macleod, Dr Megan
Authors: Hansell, C. A.H., Love, S., Pingen, M., Wilson, G. J., Macleod, M., and Graham, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:European Journal of Immunology
Publisher:Wiley
ISSN:0014-2980
ISSN (Online):1521-4141
Published Online:29 February 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in European Journal of Immunology 50(5):666-675
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
171627The ACKR2-CCR2 axis in development and diseaseGerard GrahamMedical Research Council (MRC)MR/M019764/1III - Immunology
167227Dissecting the chemokine basis for the orchestration of the in vivo inflammatory responseGerard GrahamWellcome Trust (WELLCOTR)099251/Z/12/ZIII - Immunology