Optical coherence tomography: an experimental validation for vascular imaging of saphenous vein bypass grafts

Wilson, R., Aung, H., Ju, X., Paterson, C., Sayer, R., McIntyre, A., Going, J., Irvine, R. and Berry, C. (2016) Optical coherence tomography: an experimental validation for vascular imaging of saphenous vein bypass grafts. Biomedical Physics and Engineering Express, 2(2), 025002. (doi: 10.1088/2057-1976/2/2/025002)

Abstract

Purpose. Optical coherence tomography (OCT) is a novel light-based intravascular imaging method with potential utility for quantifying vascular disease and the effect of therapy. OCT has been used to image vein grafts and neointima in clinical research studies but validity of OCT for vein graft imaging is limited by depth of field and tissue penetration. Experiments were carried out to validate the in-house developed new software (Medipass-iScan) and OCT vendor software comparing with a gold standard, photomicroscopy. Methods. Seven synthetic phantom tubes with varying inner diameters and eight saphenous veins were imaged with OCT. Imaging was performed five times on each phantom/vessel and sections of each were measured by photomicroscopy. OCT images were analyzed by iScan to measure the inner diameters and then compared with corresponding microscopy sections. Results. A Bland–Altman plot of differences between photomicroscopy and OCT measurements of phantoms, demonstrated evidence of limited bias (104 μm) and 95% limits of agreement, −100 and 308 μm. The mean variation of iScan OCT measurements from microscopy was 3.02% and that of the OCT vendor software 3.03%. Conclusions. OCT has high measurement accuracy of lumen diameter. iScan measurements of saphenous veins imaged by OCT had similar accuracy to vendor software, supporting its validity. Potentially, OCT may be used to measure saphenous vein dimensions ex vivo.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Berry, Professor Colin and Paterson, Mr Craig and Sayer, Mr Robin and Going, Dr James and Irvine, Mr Richard
• Authors: Wilson, R., Aung, H., Ju, X., Paterson, C., Sayer, R., McIntyre, A., Going, J., Irvine, R., and Berry, C.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences; College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine; College of Science and Engineering > School of Physics and Astronomy
• Journal Name: Biomedical Physics and Engineering Express
• Publisher: IOP Publishing
• ISSN: 2057-1976
• ISSN (Online): 2057-1976