Pentose phosphate metabolism in Leishmania mexicana

Maugeri, D., Cazzulo, J., Burchmore, R. , Barrett, M. and Ogbunude, P. (2003) Pentose phosphate metabolism in Leishmania mexicana. Molecular and Biochemical Parasitology, 130(2), pp. 117-125. (doi:10.1016/S0166-6851(03)00173-7)

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Abstract

The metabolism of pentose phosphates was studied in Leishmania mexicana promastigotes. Each of the enzymes of the classical pentose phosphate pathway (PPP) has been identified and specific activities measured. Functioning of the PPP was demonstrated in non-growing cells by measuring the evolution of from [1-]d-glucose and [6-]d-glucose under normal conditions and also under selective stimulation of the PPP by exposure to methylene blue. The proportion of glucose which passes through the PPP increases in the latter condition, thus suggesting a protective role against oxidant stress. The incorporation into nucleic acids of ribose 5-phosphate provided via either glucose or free ribose was also determined. Results indicate that the PPP enables glucose to serve as a source of ribose 5-phosphate in nucleotide biosynthesis. Moreover, free ribose is incorporated efficiently, implying the presence of a ribose uptake system and also of ribokinase. Ribose was shown to be accumulated by a carrier mediated process in L. mexicana promastigotes and ribokinase activity was also measured in these cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Barrett, Professor Michael
Authors: Maugeri, D., Cazzulo, J., Burchmore, R., Barrett, M., and Ogbunude, P.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier BV
ISSN:0166-6851
ISSN (Online):1872-9428

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