Proteomic analysis of mouse mammary terminal end buds identifies axonal growth cone proteins

Morris, J. , Davies, C., Griffiths, M., Page, M., Bruce, J., Patel, G., Herath, A. and Gusterson, B. (2004) Proteomic analysis of mouse mammary terminal end buds identifies axonal growth cone proteins. Proteomics, 4(6), pp. 1802-1810. (doi:10.1002/pmic.200300699)

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Abstract

Ductal morphogenesis in the mouse mammary gland occurs mainly postnatally and is driven by specialized structures at the ends of the developing ducts, the terminal end buds (TEBs), which later regress once ductal growth is complete. To identify proteins that are specifically associated with migration of TEBs we developed a novel method of isolating TEBs, which eliminated the mammary stroma. The protein expression profile of the TEBs was then compared with that of isolates taken from the 4(th) inguinal mammary gland of adult virgin mice using two-dimensional (2-D) gel electrophoresis and mass spectrometry (MS) analysis (matrix-assisted laser desorption/ionization and quadrupole time of flight). Following construction of an integrated protein expression database, 44 protein features which showed differential expression levels between the two sets were chosen for MS analysis. Of these, 24 gave protein annotations whereas the other 20 produced unidentified peptides. Fourteen unequivocal proteins were identified from these 24, whereas the remaining 10 matched more than one protein within a single 2-D gel feature. Several of the identified proteins were associated with the cytoskeleton and have previously been reported in axonal growth cones, suggesting that they may influence cell shape and motility within the advancing TEBs, in a similar fashion to migrating axons.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Morris, Professor Joanna and Gusterson, Professor Barry
Authors: Morris, J., Davies, C., Griffiths, M., Page, M., Bruce, J., Patel, G., Herath, A., and Gusterson, B.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Proteomics
ISSN:1615-9853

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