Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

McConnell, G., Smith, G. , Girkin, J., Gurney, A. and Ferguson, A. (2003) Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source. Optics Letters, 28(19), pp. 1742-1744. (doi:10.1364/OL.28.001742)

Full text not currently available from Enlighten.

Abstract

We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca2+ indicator fura-2.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Smith, Professor Godfrey
Authors: McConnell, G., Smith, G., Girkin, J., Gurney, A., and Ferguson, A.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Optics Letters
Publisher:Optical Society of America
ISSN:0146-9592
ISSN (Online):1539-4794

University Staff: Request a correction | Enlighten Editors: Update this record